No Band or Faint Band

Causes Related to Cycling Times and Temperatures: 1. Too few cycles were used - Using too few PCR cycles can lead to insufficient amplification. Use 20–35 cycles. Use fewer cycles when template concentration is high, and use more cycles when template concentration is low. 2. Extension time was too short - If the extension time is too short, there will be insufficient time for complete replication of the target. Generally, use an extension time of 1 min/kb. 3. Annealing time was too short - If the annealing time is too short, primers do not have enough time to bind to the template. Use an annealing time of at least 30 sec. #useOfPCR #PCRTest #PCRExplained #PCRAnimation #PCRProcedure #PCRCovidTest #howPcrWorks #reverseTranscriptase #cDNA #gelElectrophoresis #stepsOfPcr #annealing #denaturing #DNASynthesis #nucleotides #pcrIngredients #purposeOfPcr #PCRSequence #DNAPolymerase #TaqPolymerase #TEKS #biology #science #tutorial #overview #introduction #pcrExamples #Primer #biotechnology #dnaTechnology #cartoon #NikolaysGeneticsLesson #dnaElectrophoresis #gelElectrophoresisExplained