Top Six Effective Technique For Laboratory Sterilization & Disinfection || Shri Ram Medical College.

In order to operate with isolated cell lines, a lab must be able to sterilise its equipment and environment effectively. The medium in which your cultures are developing should not be exposed to environmental pests, and neither should the cultures themselves, therefore they must be disinfected before being thrown away. You may not realise that you have uninvited microbe guests in your cultures unless you examine them under a microbiology microscope. It’s time to refresh your memory on the numerous laboratory sterilising processes and how they protect your cells from pathogens. Using pressured steam to heat the item to be sterilised is the preferred method of sterilisation in most laboratories. Extreme heat or prolonged incubation is necessary for some bugs, but this procedure is otherwise quite successful at eliminating all types of bacteria, fungi, and viruses. Microbes are sterilised in an autoclave because the high heat and moisture effectively hydrolyze and coagulate the proteins in their cells.