Polymerase Chain Reaction (PCR): DNA Amplification

PCR is based on the mechanisms of DNA replication. First, the double-stranded DNA, which serves as the template in the reaction, is separated by heat. Next, a DNA polymerase synthesizes a new daughter strand on each single strand in one direction. By repeating these reactions continuously, a defined segment of DNA, the target, is amplified exponentially. Further information can be found in the following AMBOSS article: #xid=gp0FKS Subscribe to AMBOSS YouTube for the latest clinical examination videos, medical student interviews, study tips and tricks, and live webinars! Free 5-Day Trial: Instagram: Facebook: Twitter: #AMBOSSMed