A monohybrid cross is a mating between individuals who have different alleles at one genetic locus of interest. The character(s) being studied in a monohybrid cross are governed by two alleles for a single locus.
To carry out such a cross, each parent is chosen to be homozygous or true breeding for a given trait (locus). When a cross satisfies the conditions for a monohybrid cross, it is usually detected by a characteristic distribution of second-generation (F2) offspring that is sometimes called the monohybrid ratio.
Usage
Generally, the monohybrid cross is used to determine the F2 generation from a pair of homozygous grandparents (one grandparent dominant, the other recessive), which results in an F1 generation that are all heterozygous. Crossing two heterozygous parents from the F1 generation results in an F2 generation that produces a 75% chance for the appearance of the dominant phenotype, of which two-thirds are heterozygous, and a 25% chance for the appearance of the recessive phenotype. This cross was originally used by biologist Gregor Mendel, who crossed two pea plants to obtain a hybrid variety, discovering the possible changes in phenotypes of several types of alleles.
Mendel’s experiment
Gregor Mendel (1822--1884) was an Austrian monk who theorized basic rules of inheritance. From 1858 to 1866, he bred garden peas in his monastery garden and analyzed the offspring of these matings. The garden pea was a good choice of experimental organism because: many varieties were available that bred true for clear-cut, qualitative traits such as
seed texture (round vs wrinkled)
seed color (green vs yellow)
flower color (white vs purple)
tall vs dwarf growth habit
and three others that also varied in a qualitative - rather than quantitative - way. Peas are normally self-pollinated because the stamens and carpels are enclosed within the petals. By removing the stamens from unripe flowers, Mendel could brush pollen from another variety on the carpels when they ripened.
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