Mitosis in Mesenchymal Stem Cells using Nanolive imaging
In this video we are visualizing samples of human mesenchymal stem cells cultured with low-serum cell growth medium.
Mesenchymal Stem Cells are multipotent cells, capable to differentiate to a variety of mesenchymal tissues lineages such as cartilage, muscle, fat, bone, tendon, and stromal tissue.
A condensation of the chromatin contained in the nucleus lead to chromosome formation. This step was followed by the disappearance of the nuclear membrane, the alignment of the chromosomes in the metaphase plate, and the migration of sister chromatids to opposite ends of the cell. Two daughter cells were eventually obtained and, for each of them, chromatin went back to its relaxed state and the nuclear envelope reappeared.
Besides the division process, the protrusions observed were also of interest. There were a great number of cell-matrix and cell-cell interactions present during the mitosis as well as in the other cell appearing in the field of view.
Technology:
Nanolive imaging, Switzerland.
The 3D Cell Explorer is a high speed, high resolution and non-invasive live cell imaging microscope that can look deep inside biological systems. This allows you to record stunning 3D images of entire cells in just seconds and with a higher resolution than any conventional microscope on the market.
For more information, visit
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