VideoS2: Fluorescence live-cell imaging of cell envelope constriction in E. coli division mutants.

Three time-lapse series of each indicated E. coli mutants acquired at a 2:30 min:sec acquisition interval are shown. Bacteria were imaged at 30˚C on 1 % agarose in M9 supplemented with 0.2 % casamino acids and D-glucose. Fluorescence channels (Pal-mCherry – magenta, ZipA-sfGFP – green) were deconvolved. Video was rendered at 12 fps. Scale bar = 2 µm.