Southern blotting vs Northern blotting vs PCR

Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. ... The major difference is that RNA, rather than DNA, is analyzed in the northern blot. A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. Polymerase chain reaction (PCR) is a method widely used in molecular biology to make multiple copies of a specific DNA segment. Using PCR, a single copy (or more) of a DNA sequence is exponentially amplified to generate thousands to millions of more copies of the particular DNA segment. #polymeraseChainReaction #NorthernBlotting #SouthernBlotting #RNA #DNA #Genetics #biology