Pluripotent Stem Cells Differentiation to Pancreatic Progenitors | Protocol Preview
Watch the Full Video at
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1 Pancreatic Progenitors - a 2 minute Preview of the Experimental Protocol
Emily C. McGaugh, M. Cristina Nostro
University Health Network, Toronto General Research Institute; University Health Network, McEwen Centre for Regenerative Medicine; University of Toronto, Department of Physiology;
Here we describe a 4-stage protocol to differentiate human embryonic stem cells to NKX6-1 pancreatic progenitors in vitro. This protocol can be applied to a variety of human pluripotent stem cell lines.
Visit ?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000 videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500 institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE’s extensive library of 14,000 videos in their research,education and teaching.
Subscribe to our channel:
2 views
146
32
4 months ago 00:09:47 1
Age Reversal Pill Coming SOON? David Sinclair’s NEW RESEARCH
8 months ago 00:02:45 1
ETHICAL CONCERNS ON STEM CELLS
8 months ago 00:00:00 1
ISS National Lab Prelaunch Science Webinar: NASA’s SpaceX CRS-30 (Mar. 8, 2024)
8 months ago 00:11:16 1
Top 10 Groundbreaking Scientific Discoveries of the 21st Century
11 months ago 00:54:57 1
Rebuilding the kidney from stem cells
11 months ago 00:02:01 2
Pluripotent Stem Cells Differentiation to Pancreatic Progenitors | Protocol Preview
11 months ago 00:02:38 5
’Artificial embryos’: the hidden steps in forming a spine
1 year ago 00:30:00 1
Jurgen Knoblich (IMBA) 2: Modeling Human Brain Development in 3D Organoid Culture
1 year ago 00:32:58 1
Arnold Kriegstein (UCSF) 2: Cerebral Organoids: Models of Human Brain Disease and Evolution
1 year ago 00:31:05 1
Arnold Kriegstein (UCSF) 1: Outer Subventricular Zone Radial Glia Cells - Brain Development
1 year ago 00:06:36 4
cellF - Video Documentation
1 year ago 00:07:52 1
This technology will change AI (and us)
1 year ago 00:03:36 1
How to Maintain and Assess Morphology of Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
1 year ago 00:05:21 1
How to Coat Plates for Human Pluripotent Stem Cell (hPSC) Cultures in mTeSR™ Plus
1 year ago 00:07:58 1
How to Generate Cell Aggregates and Passage Human Pluripotent Stem Cells (hPSCs) in mTeSR™ Plus
1 year ago 00:01:17 1
Tapping Technique for Passaging Pluripotent Stem Cells Using ReLeSR™ Selection and Passaging Reagent
1 year ago 00:01:30 1
How to Transition Human Pluripotent Stem Cells into mTeSR™ Plus from Other Feeder-Free Media
1 year ago 00:02:22 1
Making Pluripotent Stem Cells
1 year ago 00:04:50 1
What is a pluripotent stem cell?
1 year ago 00:02:06 1
How to Count 3D Sphere Cultures (Mammospheres, Neurospheres, Cancer Spheroids, and PSC Aggregates)
2 years ago 00:02:57 1
Sample - Generation of Teeth Using Stem Cells
2 years ago 01:06:10 3
Modeling the human brain using induced pluripotent stem cells iPSCs
2 years ago 00:02:22 1
Madeline Lancaster, PhD, 2022 Blavatnik Awards in the UK Laureate in Life Sciences